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floA [2019-04-15 18:58:50]
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floA [2019-04-15 18:58:50]

flottilin-like protein (in addition to [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|FloT]), resistence protein (against sublancin), accessory role in resistance to cefuroxime
locus
BSU25380
pI
4.92
mw
35.48 kDa
protein length
331 aa Sequence Blast
gene length
996 bp Sequence Blast
function
resistance against sublancin
product
flottilin-like protein
essential
no
synonyms
yqfA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,617,449 2,618,444

    Phenotypes of a mutant

  • reduced [SW|protein secretion] [Pubmed|23651456]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant does not induce [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
  • a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|22882210]
  • a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
  • a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]

    • The protein

    Catalyzed reaction/ biological activity

  • controls protease activity of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|24222488]

    • Protein family

  • UPF0365 family (according to Swiss-Prot)

    • [SW|Localization]

  • membrane [Pubmed|18763711]
  • co-localizes with [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|FloT] in discrete foci in the membrane [Pubmed|20713508]
  • forms focal structure in the cytoplasma membrane [Pubmed|22753055]
  • 13 foci [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|FloA] of are detected in strain 3610 [Pubmed|25909364]

    • Expression and Regulation

    Operons

    genes
    [gene|F6B0D27B434D81B7A680555E4457F266B6206B7D|yqeZ]-[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]-[gene|A80317C3B8DF06E2187E02A05C13845508C18B40|yqfB]
    description
    [Pubmed|11866510]

    sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|11866510], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

    • regulation

  • expressed under stress conditions ([protein|search|SigW]) [Pubmed|11866510]
    • view in new tab

    additional information

  • the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

    • Biological materials

    Mutant

  • MGNA-C443 (yqfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2441 NBRP B. subtilis, Japan]
  • JS119 (3610 markerless), available in [SW|Daniel Lopez]'s lab
  • DL1401 (168 ''floA''::mls), available in [SW|Daniel Lopez]'s lab
  • BKE25380 ([gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG
  • BKK25380 ([gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG

    • GFP fusion

  • GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [SW|Daniel Lopez]'s lab
  • JS136 (3610 ''amyE::floT''-gfp(spc)), available in [SW|Daniel Lopez]'s lab
  • JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
  • JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloA [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab

    • References

    Reviews

  • 25652542

    • Original publications

  • 16629676,11866510,18763711,20713508,22753055,22882210,23651456,22178969,24222488,25909364,26297017,27362352,26883633